{"entity": "journal", "iuid": "26167cb1db424e04b4c36c7e6fa0a5a0", "timestamp": "2026-08-20T20:36:31.806Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Biosensors%20%28Basel%29.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Biosensors%20%28Basel%29"}}, "title": "Biosensors (Basel)", "issn": "2079-6374", "issn-l": null, "publications_count": 4, "publications": [{"entity": "publication", "iuid": "21b2fba629b34393bd8e2054d8099d6c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/21b2fba629b34393bd8e2054d8099d6c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/21b2fba629b34393bd8e2054d8099d6c"}}, "title": "Regulatory Effects of RNA-Protein Interactions Revealed by Reporter Assays of Bacteria Grown on Solid Media.", "authors": [{"family": "P\u00e9rez-Ropero", "given": "Guillermo", "initials": "G", "orcid": "0000-0001-7595-4851", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3ab708040fd948028309564a02b365d1.json"}}, {"family": "Dolcemascolo", "given": "Roswitha", "initials": "R", "orcid": "0000-0003-2851-7194", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b7bc348a827a4e6ab3ff82f1e2c5925b.json"}}, {"family": "P\u00e9rez-R\u00e0fols", "given": "Anna", "initials": "A", "orcid": "0000-0001-7492-2583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5a6fa8b62a9d4bcaa73ea3e19256b533.json"}}, {"family": "Andersson", "given": "Karl", "initials": "K", "orcid": "0000-0001-9141-9242", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3c2790814ce34e97b517ace827470e6d.json"}}, {"family": "Danielson", "given": "U Helena", "initials": "UH", "orcid": "0000-0003-2728-0340", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1be15d9a1ea49e1b0e7bb712522696e.json"}}, {"family": "Rodrigo", "given": "Guillermo", "initials": "G", "orcid": "0000-0002-1871-9617", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/194cb38da72a4278a5a84ea87580f288.json"}}, {"family": "Buijs", "given": "Jos", "initials": "J", "orcid": "0000-0003-3955-1320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b8ab0f168ed348baa89d0578fc9c78a1.json"}}], "type": "journal article", "published": "2025-03-08", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "15", "issue": "3", "issn-l": null}, "abstract": "Reporter systems are widely used to study biomolecular interactions and processes in vivo, representing one of the basic tools used to characterize synthetic regulatory circuits. Here, we developed a method that enables the monitoring of RNA-protein interactions through a reporter system in bacteria with high temporal resolution. For this, we used a Real-Time Protein Expression Assay (RT-PEA) technology for real-time monitoring of a fluorescent reporter protein, while having bacteria growing on solid media. Experimental results were analyzed by fitting a three-variable Gompertz growth model. To validate the method, the interactions between a set of RNA sequences and the RNA-binding protein (RBP) Musashi-1 (MSI1) were evaluated, as well as the allosteric modulation of the interaction by a small molecule (oleic acid). This new approach proved to be suitable to quantitatively characterize RNA-RBP interactions, thereby expanding the toolbox to study molecular interactions in living bacteria, including allosteric modulation, with special relevance for systems that are not suitable to be studied in liquid media.", "doi": "10.3390/bios15030175", "pmid": "40136972", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11940492"}, {"db": "pii", "key": "bios15030175"}], "notes": [], "created": "2026-08-20T13:39:06.008Z", "modified": "2026-08-20T13:39:06.236Z"}, {"entity": "publication", "iuid": "71734534a2814d04b69dd990d234cdc9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/71734534a2814d04b69dd990d234cdc9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/71734534a2814d04b69dd990d234cdc9"}}, "title": "Metabolic Assessment of Human Induced Pluripotent Stem Cells-Derived Astrocytes and Fetal Primary Astrocytes: Lactate and Glucose Turnover.", "authors": [{"family": "Matthiesen", "given": "Isabelle", "initials": "I"}, {"family": "Nasiri", "given": "Rohollah", "initials": "R", "orcid": "0000-0002-8245-692X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aaecdaea25fc438e937adfea37188ae1.json"}}, {"family": "Tamashiro Orrego", "given": "Alessandra", "initials": "A"}, {"family": "Winkler", "given": "Thomas E", "initials": "TE", "orcid": "0000-0002-2331-4833", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/851879d753574f5a80716e94dcc24590.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2022-10-08", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "12", "issue": "10", "issn-l": null}, "abstract": "Astrocytes represent one of the main cell types in the brain and play a crucial role in brain functions, including supplying the energy demand for neurons. Moreover, they are important regulators of metabolite levels. Glucose uptake and lactate production are some of the main observable metabolic actions of astrocytes. To gain insight into these processes, it is essential to establish scalable and functional sources for in vitro studies of astrocytes. In this study, we compared the metabolic turnover of glucose and lactate in astrocytes derived from human induced pluripotent stem cell (hiPSC)-derived Astrocytes (hiAstrocytes) as a scalable astrocyte source to human fetal astrocytes (HFAs). Using a user-friendly, commercial flow-based biosensor, we could verify that hiAstrocytes are as glycogenic as their fetal counterparts, but their normalized metabolic turnover is lower. Specifically, under identical culture conditions in a defined media, HFAs have 2.3 times higher levels of lactate production compared to hiAstrocytes. In terms of glucose, HFAs have 2.1 times higher consumption levels than hiAstrocytes at 24 h. Still, as we describe their glycogenic phenotype, our study demonstrates the use of hiAstrocytes and flow-based biosensors for metabolic studies of astrocyte function.", "doi": "10.3390/bios12100839", "pmid": "36290976", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9599592"}, {"db": "pii", "key": "bios12100839"}], "notes": [], "created": "2026-08-20T13:39:00.656Z", "modified": "2026-08-20T13:39:00.766Z"}, {"entity": "publication", "iuid": "89f9b2c01cbd487cbf38391bc959dbad", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/89f9b2c01cbd487cbf38391bc959dbad.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/89f9b2c01cbd487cbf38391bc959dbad"}}, "title": "3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures.", "authors": [{"family": "Khati", "given": "Vamakshi", "initials": "V"}, {"family": "Ramachandraiah", "given": "Harisha", "initials": "H"}, {"family": "Pati", "given": "Falguni", "initials": "F", "orcid": "0000-0002-3588-1800", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a980f4446de44c9ca89931a1c774f1e6.json"}}, {"family": "Svahn", "given": "Helene A", "initials": "HA"}, {"family": "Gaudenzi", "given": "Giulia", "initials": "G"}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2022-07-13", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "12", "issue": "7", "issn-l": null}, "abstract": "Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 \u00b0C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s-1) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85-93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures.", "doi": "10.3390/bios12070521", "pmid": "35884324", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9313433"}, {"db": "pii", "key": "bios12070521"}], "notes": [], "created": "2026-08-20T13:38:58.648Z", "modified": "2026-08-20T13:38:58.770Z"}, {"entity": "publication", "iuid": "18477e59733745b1b2c909f841947110", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/18477e59733745b1b2c909f841947110.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/18477e59733745b1b2c909f841947110"}}, "title": "Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay.", "authors": [{"family": "Sepehri", "given": "Sobhan", "initials": "S", "orcid": "0000-0002-2145-1340", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b520c2f4105e45e384cb73e7ae90506f.json"}}, {"family": "Agnarsson", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Torre", "given": "Teresa Zard\u00e1n G\u00f3mez de la", "initials": "TZG"}, {"family": "Schneiderman", "given": "Justin F", "initials": "JF"}, {"family": "Blomgren", "given": "Jakob", "initials": "J", "orcid": "0000-0002-6148-3574", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58c6f299960946c08ccdfded2fd000fb.json"}}, {"family": "Jesorka", "given": "Aldo", "initials": "A"}, {"family": "Johansson", "given": "Christer", "initials": "C"}, {"family": "Nilsson", "given": "Mats", "initials": "M"}, {"family": "Albert", "given": "Jan", "initials": "J"}, {"family": "Str\u00f8mme", "given": "Maria", "initials": "M"}, {"family": "Winkler", "given": "Dag", "initials": "D"}, {"family": "Kalaboukhov", "given": "Alexei", "initials": "A"}], "type": "journal article", "published": "2019-09-17", "journal": {"title": "Biosensors (Basel)", "issn": "2079-6374", "volume": "9", "issue": "3", "issn-l": null}, "abstract": "The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.", "doi": "10.3390/bios9030109", "pmid": "31533330", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6784358"}, {"db": "pii", "key": "bios9030109"}], "notes": [], "created": "2026-08-20T13:39:08.069Z", "modified": "2026-08-20T13:39:08.186Z"}], "created": "2026-08-20T13:38:58.693Z", "modified": "2026-08-20T13:38:58.693Z"}