{"entity": "journal", "iuid": "9f2d7a4170264807878e8991053d7c1c", "timestamp": "2026-08-20T20:45:13.353Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Acta%20Neuropathol%20Commun.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Acta%20Neuropathol%20Commun"}}, "title": "Acta Neuropathol Commun", "issn": "2051-5960", "issn-l": null, "publications_count": 3, "publications": [{"entity": "publication", "iuid": "c6e22a4518904c91b2195081c97bc90c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c6e22a4518904c91b2195081c97bc90c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c6e22a4518904c91b2195081c97bc90c"}}, "title": "Decoding of the surfaceome and endocytome in primary glioblastoma cells identifies potential target antigens in the hypoxic tumor niche.", "authors": [{"family": "de Oliveira", "given": "Kelin Gon\u00e7alves", "initials": "KG"}, {"family": "B\u00e5ng-Rudenstam", "given": "Anna", "initials": "A"}, {"family": "Beyer", "given": "Sarah", "initials": "S"}, {"family": "Boukredine", "given": "Axel", "initials": "A"}, {"family": "Talbot", "given": "Hugo", "initials": "H"}, {"family": "Governa", "given": "Valeria", "initials": "V"}, {"family": "Johansson", "given": "Maria C", "initials": "MC"}, {"family": "M\u00e5nsson", "given": "Ann-Sofie", "initials": "AS"}, {"family": "Forsberg-Nilsson", "given": "Karin", "initials": "K"}, {"family": "Bengzon", "given": "Johan", "initials": "J"}, {"family": "Malmstr\u00f6m", "given": "Johan", "initials": "J"}, {"family": "Welinder", "given": "Charlotte", "initials": "C"}, {"family": "Belting", "given": "Mattias", "initials": "M", "orcid": "0000-0003-1585-5434", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d9763a47486d410fb5034fb2603f3a02.json"}}], "type": "journal article", "published": "2024-02-27", "journal": {"title": "Acta Neuropathol Commun", "issn": "2051-5960", "volume": "12", "issue": "1", "pages": "35", "issn-l": null}, "abstract": "Immunotherapies with antibody-drug-conjugates (ADC) and CAR-T cells, targeted at tumor surface antigens (surfaceome), currently revolutionize clinical oncology. However, target identification warrants a better understanding of the surfaceome and how it is modulated by the tumor microenvironment. Here, we decode the surfaceome and endocytome and its remodeling by hypoxic stress in glioblastoma (GBM), the most common and aggressive brain tumor in adults. We employed a comprehensive approach for global and dynamic profiling of the surfaceome and endocytosed (endocytome) proteins and their regulation by hypoxia in patient-derived GBM cultures. We found a heterogeneous surface-endocytome profile and a divergent response to hypoxia across GBM cultures. We provide a quantitative ranking of more than 600 surface resident and endocytosed proteins, and their regulation by hypoxia, serving as a resource to the cancer research community. As proof-of-concept, the established target antigen CD44 was identified as a commonly and abundantly expressed surface protein with high endocytic activity. Among hypoxia induced proteins, we reveal CXADR, CD47, CD81, BSG, and FXYD6 as potential targets of the stressed GBM niche. We could validate these findings by immunofluorescence analyses in patient tumors and by increased expression in the hypoxic core of GBM spheroids. Selected candidates were finally confronted by treatment studies, showing their high capacity for internalization and ADC delivery. Importantly, we highlight the limited correlation between transcriptomics and proteomics, emphasizing the critical role of membrane protein enrichment strategies and quantitative mass spectrometry. Our findings provide a comprehensive understanding of the surface-endocytome and its remodeling by hypoxia in GBM as a resource for exploration of targets for immunotherapeutic approaches in GBM.", "doi": "10.1186/s40478-024-01740-z", "pmid": "38414005", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10898066"}, {"db": "pii", "key": "10.1186/s40478-024-01740-z"}], "notes": [], "created": "2026-08-20T12:37:07.397Z", "modified": "2026-08-20T12:37:07.420Z"}, {"entity": "publication", "iuid": "5dbb263969684a2f8c53b25e6362650c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5dbb263969684a2f8c53b25e6362650c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5dbb263969684a2f8c53b25e6362650c"}}, "title": "Annotation of pituitary neuroendocrine tumors with genome-wide expression analysis.", "authors": [{"family": "Tebani", "given": "Abdellah", "initials": "A"}, {"family": "Jotanovic", "given": "Jelena", "initials": "J"}, {"family": "Hekmati", "given": "Neda", "initials": "N"}, {"family": "Sivertsson", "given": "\u00c5sa", "initials": "\u00c5"}, {"family": "Gudjonsson", "given": "Olafur", "initials": "O"}, {"family": "Ed\u00e9n Engstr\u00f6m", "given": "Britt", "initials": "B"}, {"family": "Wikstr\u00f6m", "given": "Johan", "initials": "J"}, {"family": "Uhl\u00e8n", "given": "Mathias", "initials": "M"}, {"family": "Casar-Borota", "given": "Olivera", "initials": "O", "orcid": "0000-0001-7376-7331", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be2f108c23f24ce6b661c0446eeb3134.json"}}, {"family": "Pont\u00e9n", "given": "Fredrik", "initials": "F"}], "type": "journal article", "published": "2021-11-10", "journal": {"title": "Acta Neuropathol Commun", "issn": "2051-5960", "volume": "9", "issue": "1", "pages": "181", "issn-l": null}, "abstract": "Pituitary neuroendocrine tumors (PitNETs) are common, generally benign tumors with complex clinical characteristics related to hormone hypersecretion and/or growing sellar tumor mass. PitNETs can be classified based on the expression pattern of anterior pituitary hormones and three main transcriptions factors (TF), SF1, PIT1 and TPIT that regulate differentiation of adenohypophysial cells. Here, we have extended this classification based on the global transcriptomics landscape using tumor tissue from a well-defined cohort comprising 51 PitNETs of different clinical and histological types. The molecular profiles were compared with current classification schemes based on immunohistochemistry. Our results identified three main clusters of PitNETs that were aligned with the main pituitary TFs expression patterns. Our analyses enabled further identification of specific genes and expression patterns, including both known and unknown genes, that could distinguish the three different classes of PitNETs. We conclude that the current classification of PitNETs based on the expression of SF1, PIT1 and TPIT reflects three distinct subtypes of PitNETs with different underlying biology and partly independent from the expression of corresponding hormones. The transcriptomic analysis reveals several potentially targetable tumor-driving genes with previously unknown role in pituitary tumorigenesis.", "doi": "10.1186/s40478-021-01284-6", "pmid": "34758873", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8579660"}, {"db": "pii", "key": "10.1186/s40478-021-01284-6"}], "notes": [], "created": "2026-08-20T12:37:05.594Z", "modified": "2026-08-20T12:37:05.650Z"}, {"entity": "publication", "iuid": "ed02e9501d2945cda4aff28bc95ef85e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ed02e9501d2945cda4aff28bc95ef85e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ed02e9501d2945cda4aff28bc95ef85e"}}, "title": "Heparanase overexpression impedes perivascular clearance of amyloid-\u03b2 from murine brain: relevance to Alzheimer's disease.", "authors": [{"family": "Zhang", "given": "Xiao", "initials": "X"}, {"family": "O'Callaghan", "given": "Paul", "initials": "P"}, {"family": "Li", "given": "Honglian", "initials": "H"}, {"family": "Tan", "given": "Yingxia", "initials": "Y"}, {"family": "Zhang", "given": "Ganlin", "initials": "G"}, {"family": "Barash", "given": "Uri", "initials": "U"}, {"family": "Wang", "given": "Xiaomin", "initials": "X"}, {"family": "Lannfelt", "given": "Lars", "initials": "L"}, {"family": "Vlodavsky", "given": "Israel", "initials": "I"}, {"family": "Lindahl", "given": "Ulf", "initials": "U"}, {"family": "Li", "given": "Jin-Ping", "initials": "JP"}], "type": "journal article", "published": "2021-05-10", "journal": {"title": "Acta Neuropathol Commun", "issn": "2051-5960", "volume": "9", "issue": "1", "pages": "84", "issn-l": null}, "abstract": "Defective amyloid-\u03b2 (A\u03b2) clearance from the brain is a major contributing factor to the pathophysiology of Alzheimer's disease (AD). A\u03b2 clearance is mediated by macrophages, enzymatic degradation, perivascular drainage along the vascular basement membrane (VBM) and transcytosis across the blood-brain barrier (BBB). AD pathology is typically associated with cerebral amyloid angiopathy due to perivascular accumulation of A\u03b2. Heparan sulfate (HS) is an important component of the VBM, thought to fulfill multiple roles in AD pathology. We previously showed that macrophage-mediated clearance of intracortically injected A\u03b2 was impaired in the brains of transgenic mice overexpressing heparanase (Hpa-tg). This study revealed that perivascular drainage was impeded in the Hpa-tg brain, evidenced by perivascular accumulation of the injected A\u03b2 in the thalamus of Hpa-tg mice. Furthermore, endogenous A\u03b2 accumulated at the perivasculature of Hpa-tg thalamus, but not in control thalamus. This perivascular clearance defect was confirmed following intracortical injection of dextran that was largely retained in the perivasculature of Hpa-tg brains, compared to control brains. Hpa-tg brains presented with thicker VBMs and swollen perivascular astrocyte endfeet, as well as elevated expression of the BBB-associated water-pump protein aquaporin 4 (AQP4). Elevated levels of both heparanase and AQP4 were also detected in human AD brain. These findings indicate that elevated heparanase levels alter the organization and composition of the BBB, likely through increased fragmentation of BBB-associated HS, resulting in defective perivascular drainage. This defect contributes to perivascular accumulation of A\u03b2 in the Hpa-tg brain, highlighting a potential role for heparanase in the pathogenesis of AD.", "doi": "10.1186/s40478-021-01182-x", "pmid": "33971986", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8111754"}, {"db": "pii", "key": "10.1186/s40478-021-01182-x"}], "notes": [], "created": "2026-08-20T12:36:59.139Z", "modified": "2026-08-20T12:36:59.219Z"}], "created": "2026-08-20T12:36:59.171Z", "modified": "2026-08-20T12:36:59.171Z"}