{"entity": "journal", "iuid": "1d0b585950734ab9b889b57e1397aca7", "timestamp": "2026-05-21T02:26:59.298Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/Acta%20Biomater.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/Acta%20Biomater"}}, "title": "Acta Biomater", "issn": "1878-7568", "issn-l": "1742-7061", "publications_count": 4, "publications": [{"entity": "publication", "iuid": "d97df535a30c4a789ae7f424827076ac", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d97df535a30c4a789ae7f424827076ac.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d97df535a30c4a789ae7f424827076ac"}}, "title": "Bioinspired coupled helical coils for soft tissue engineering of tubular structures - Improved mechanical behavior of tubular collagen type I templates.", "authors": [{"family": "Janke", "given": "H P", "initials": "HP"}, {"family": "Bohlin", "given": "J", "initials": "J"}, {"family": "Lomme", "given": "R M L M", "initials": "RMLM"}, {"family": "Mihaila", "given": "S M", "initials": "SM"}, {"family": "Hilborn", "given": "J", "initials": "J"}, {"family": "Feitz", "given": "W F J", "initials": "WFJ"}, {"family": "Oosterwijk", "given": "E", "initials": "E"}], "type": "journal article", "published": "2017-09-01", "journal": {"title": "Acta Biomater", "issn": "1878-7568", "volume": "59", "issue": null, "pages": "234-242", "issn-l": "1742-7061"}, "abstract": "The design of constructs for tubular tissue engineering is challenging. Most biomaterials need to be reinforced with supporting structures such as knittings, meshes or electrospun material to comply with the mechanical demands of native tissues. In this study, coupled helical coils (CHCs) were manufactured to mimic collagen fiber orientation as found in nature. Monofilaments of different commercially available biodegradable polymers were wound and subsequently fused, resulting in right-handed and left-handed polymer helices fused together in joints where the filaments cross. CHCs of different polymer composition were tested to determine the tensile strength, strain recovery, hysteresis, compressive strength and degradation of CHCs of different composition. Subsequently, seamless and stable hybrid constructs consisting of PDSII\u00ae USP 2-0 CHCs embedded in porous collagen type I were produced. Compared to collagen alone, this hybrid showed superior strain recovery (93.5\u00b10.9% vs 71.1\u00b112.6% in longitudinal direction; 87.1\u00b16.6% vs 57.2\u00b14.6% in circumferential direction) and hysteresis (18.9\u00b12.7% vs 51.1\u00b112.0% in longitudinal direction; 11.5\u00b14.6% vs 46.3\u00b16.3% in circumferential direction). Furthermore, this hybrid construct showed an improved Young's modulus in both longitudinal (0.5\u00b10.1MPavs 0.2\u00b10.1MPa; 2.5-fold) and circumferential (1.65\u00b10.07MPavs (2.9\u00b10.3)\u00d710\n\nMost tubular constructs lack sufficient strength and tunability to comply with the mechanical demands of native tissues. Therefore, we embedded coupled helical coils (CHCs) produced from biodegradable polymers - to mimic collagen fiber orientation as found in nature - in collagen type I sponges. We show that the mechanical behavior of CHCs is very similar to native tissue and strengths structurally weak tubular constructs. The production procedure is relatively easy, reproducible and mechanical features can be controlled to meet different mechanical demands. This is promising in template manufacture, hence offering new opportunities in tissue engineering of tubular organs and preventing graft failure.", "doi": "10.1016/j.actbio.2017.06.038", "pmid": "28666884", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(17)30420-8"}], "notes": [], "created": "2018-12-05T12:09:41.549Z", "modified": "2018-12-05T12:09:41.567Z"}, {"entity": "publication", "iuid": "7f896353d5204b3a83ae91086b934b59", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7f896353d5204b3a83ae91086b934b59.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7f896353d5204b3a83ae91086b934b59"}}, "title": "Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.", "authors": [{"family": "Zhang", "given": "Yu", "initials": "Y"}, {"family": "Heher", "given": "Philipp", "initials": "P"}, {"family": "Hilborn", "given": "J\u00f6ns", "initials": "J"}, {"family": "Redl", "given": "Heinz", "initials": "H"}, {"family": "Ossipov", "given": "Dmitri A", "initials": "DA"}], "type": "journal article", "published": "2016-07-01", "journal": {"title": "Acta Biomater", "issn": "1878-7568", "volume": "38", "issue": null, "pages": "23-32", "issn-l": "1742-7061"}, "abstract": "To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking. The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups. Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks. The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components. The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts. The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels. Together with the persistence of HA network, it permitted longer cell culturing time in the IPN. Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues. These results are promising for tunable ECM-based materials for TE and regenerative medicine.\n\nThe present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other. This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA. High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells. The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network. As a result, the cells have sufficient time for the remodelling of the scaffold. This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.", "doi": "10.1016/j.actbio.2016.04.041", "pmid": "27134013", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(16)30203-3"}], "notes": [], "created": "2018-12-05T09:57:41.070Z", "modified": "2018-12-05T09:57:41.088Z"}, {"entity": "publication", "iuid": "ee8a80d86f764544a2091e395827840c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ee8a80d86f764544a2091e395827840c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ee8a80d86f764544a2091e395827840c"}}, "title": "Non-invasive in vitro and in vivo monitoring of degradation of fluorescently labeled hyaluronan hydrogels for tissue engineering applications.", "authors": [{"family": "Zhang", "given": "Yu", "initials": "Y"}, {"family": "Rossi", "given": "Filippo", "initials": "F"}, {"family": "Papa", "given": "Simonetta", "initials": "S"}, {"family": "Violatto", "given": "Martina Bruna", "initials": "MB"}, {"family": "Bigini", "given": "Paolo", "initials": "P"}, {"family": "Sorbona", "given": "Marco", "initials": "M"}, {"family": "Redaelli", "given": "Francesca", "initials": "F"}, {"family": "Veglianese", "given": "Pietro", "initials": "P"}, {"family": "Hilborn", "given": "J\u00f6ns", "initials": "J"}, {"family": "Ossipov", "given": "Dmitri A", "initials": "DA"}], "type": "journal article", "published": "2016-01-00", "journal": {"title": "Acta Biomater", "issn": "1878-7568", "volume": "30", "issue": null, "pages": "188-198", "issn-l": "1742-7061"}, "abstract": "Tracking of degradation of hydrogels-based biomaterials in vivo is very important for rational design of tissue engineering scaffolds that act as delivery carriers for bioactive factors. During the process of tissue development, an ideal scaffold should remodel at a rate matching with scaffold degradation. To reduce amount of animals sacrificed, non-invasive in vivo imaging of biomaterials is required which relies on using of biocompatible and in situ gel forming compounds carrying suitable imaging agents. In this study we developed a method of in situ fabrication of fluorescently labeled and injectable hyaluronan (HA) hydrogel based on one pot sequential use of Michael addition and thiol-disulfide exchange reactions for the macromolecules labeling and cross-linking respectively. Hydrogels with different content of HA were prepared and their enzymatic degradation was followed in vitro and in vivo using fluorescence multispectral imaging. First, we confirmed that the absorbance of the matrix-linked near-IR fluorescent IRDye\u00ae 800CW agent released due to the matrix enzymatic degradation in vitro matched the amount of the degraded hydrogel measured by classical gravimetric method. Secondly, the rate of degradation was inversely proportional to the hydrogel concentration and this structure-degradation relationship was similar for both in vitro and in vivo studies. It implies that the degradation of this disulfide cross-linked hyaluronan hydrogel in vivo can be predicted basing on the results of its in vitro degradation studies. The compliance of in vitro and in vivo methods is also promising for the future development of predictive in vitro tissue engineering models.\n\nThe need for engineered hydrogel scaffolds that deliver bioactive factors to endogenous progenitor cells in vivo via gradual matrix resorption and thus facilitate tissue regeneration is increasing with the aging population. Importantly, scaffold should degrade at a modest rate that will not be too fast to support tissue growth nor too slow to provide space for tissue development. The present work is devoted to longitudinal tracking of a hydrogel material in vivo from the time of its implantation to the time of complete resorption without sacrificing animals. The method demonstrates correlation of resorption rates in vivo and in vitro for hydrogels with varied structural parameters. It opens the possibility to develop predictive in vitro models for tissue engineered scaffolds and reduce animal studies.", "doi": "10.1016/j.actbio.2015.11.053", "pmid": "26621694", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(15)30229-4"}], "notes": [], "created": "2018-12-05T09:24:11.333Z", "modified": "2018-12-05T09:24:11.354Z"}, {"entity": "publication", "iuid": "e4a642f235f64813ad8efa513403ae05", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e4a642f235f64813ad8efa513403ae05.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e4a642f235f64813ad8efa513403ae05"}}, "title": "Investigation of the three-dimensional orientation of mineralized collagen fibrils in human lamellar bone using synchrotron X-ray phase nano-tomography.", "authors": [{"family": "Varga", "given": "Peter", "initials": "P"}, {"family": "Pacureanu", "given": "Alexandra", "initials": "A"}, {"family": "Langer", "given": "Max", "initials": "M"}, {"family": "Suhonen", "given": "Heikki", "initials": "H"}, {"family": "Hesse", "given": "Bernhard", "initials": "B"}, {"family": "Grimal", "given": "Quentin", "initials": "Q"}, {"family": "Cloetens", "given": "Peter", "initials": "P"}, {"family": "Raum", "given": "Kay", "initials": "K"}, {"family": "Peyrin", "given": "Fran\u00e7oise", "initials": "F"}], "type": "journal article", "published": "2013-09-00", "journal": {"title": "Acta Biomater", "issn": "1878-7568", "volume": "9", "issue": "9", "pages": "8118-8127", "issn-l": "1742-7061"}, "abstract": "We investigate the three-dimensional (3-D) organization of mineralized collagen fibrils in human cortical bone based on synchrotron X-ray phase nano-tomography images. In lamellar bone the collagen fibrils are assumed to have a plywood-like arrangement, but due to experimental limitations the 3-D fibril structure has only been deduced from section surfaces so far and the findings have been controversial. Breakthroughs in synchrotron tomographic imaging have given access to direct 3-D information on the bone structure at the nanoscale level. Using an autocorrelation-based orientation measure we confirm that the fibrils are unidirectional in quasi-planes of sub-lamellae and find two specific dominant patterns, oscillating and twisted plywoods coexisting in a single osteon. Both patterns exhibit smooth orientation changes between adjacent quasi-planes. Moreover, we find that the periodic changes in collagen fibril orientation are independent of fluctuations in local mass density. These data improve our understanding of the lamellar arrangement in bone and allow more detailed investigations of structure-function relationships at this scale, providing templates for bio-inspired materials. The presented methodology can be applied to non-destructive 3-D characterization of the sub-micron scale structure of other natural and artificial mineralized biomaterials. ", "doi": "10.1016/j.actbio.2013.05.015", "pmid": "23707503", "labels": {"Affiliated researcher": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(13)00248-1"}], "notes": [], "created": "2018-12-05T09:03:01.497Z", "modified": "2018-12-05T09:03:01.530Z"}], "created": "2018-12-05T09:03:01.511Z", "modified": "2020-11-27T13:12:57.893Z"}