{"entity": "journal", "iuid": "a3210379cec340d49a726fe6f8348267", "timestamp": "2026-09-06T14:43:30.866Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/journal/ACS%20Appl%20Mater%20Interfaces.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/journal/ACS%20Appl%20Mater%20Interfaces"}}, "title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "issn-l": "1944-8244", "publications_count": 10, "publications": [{"entity": "publication", "iuid": "09baeb2926c4435d9f51285afdea06bb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/09baeb2926c4435d9f51285afdea06bb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/09baeb2926c4435d9f51285afdea06bb"}}, "title": "Sub-5 nm Silicon Nanopore Sensors: Scalable Fabrication via Self-Limiting Metal-Assisted Chemical Etching.", "authors": [{"family": "De Ferrari", "given": "Fabio", "initials": "F", "orcid": "0000-0003-0960-9931", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21be7bf528794d57b0b1431da2a95247.json"}}, {"family": "Raja", "given": "Shyamprasad N", "initials": "SN", "orcid": "0000-0002-2278-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58df34262ccf4569b54162cfd38525be.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}], "type": "journal article", "published": "2025-02-12", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "17", "issue": "6", "pages": "9047-9058", "issn-l": "1944-8244"}, "abstract": "Solid-state nanopores offer unique possibilities for biomolecule sensing; however, scalable production of sub-5 nm pores with precise diameter control remains a manufacturing challenge. In this work, we developed a scalable method to fabricate sub-5 nm nanopores in silicon (Si) nanomembranes through metal-assisted chemical etching (MACE) using gold nanoparticles. Notably, we present a previously unreported self-limiting effect that enables sub-5 nm nanopore formation from both 10 and 40 nm nanoparticles in the 12 nm thick monocrystalline device layer of a silicon-on-insulator substrate. This effect reveals distinctive etching dynamics in ultrathin Si nanomembranes, enabling precise control over nanopore dimensions. The resulting nanopore sensor, suspended over self-aligned spheroidal oxide undercuts with diameters of just a few hundred nanometers, exhibited low electrical noise and high stability due to encapsulation within dielectric layers. In DNA translocation experiments, our nanopore platform could distinguish folded and unfolded DNA conformations and maintained stable baseline conductance for up to 6 h, demonstrating both sensitivity and robustness. Our scalable nanopore fabrication method is compatible with wafer-level and batch processing and holds promise for advancing biomolecular sensing and analysis.", "doi": "10.1021/acsami.4c19750", "pmid": "39882662", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11826499"}], "notes": [], "created": "2026-08-20T08:08:23.883Z", "modified": "2026-08-21T11:34:54.511Z"}, {"entity": "publication", "iuid": "7d836b85756546f1b18f2851412b8d7e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7d836b85756546f1b18f2851412b8d7e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7d836b85756546f1b18f2851412b8d7e"}}, "title": "Localized Nanopore Fabrication in Silicon Nitride Membranes by Femtosecond Laser Exposure and Subsequent Controlled Breakdown.", "authors": [{"family": "Leva", "given": "Chrysovalantou V", "initials": "CV"}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Kistermann", "given": "Kevin", "initials": "K"}, {"family": "Sakurai", "given": "Kasumi", "initials": "K"}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Mayer", "given": "Joachim", "initials": "J", "orcid": "0000-0003-3292-5342", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6b355f200cc346b389e9dc7901872fe7.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}, {"family": "Raja", "given": "Shyamprasad N", "initials": "SN", "orcid": "0000-0002-2278-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58df34262ccf4569b54162cfd38525be.json"}}], "type": "journal article", "published": "2025-02-05", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "17", "issue": "5", "pages": "8737-8748", "issn-l": "1944-8244"}, "abstract": "Controlled breakdown has emerged as an effective method for fabricating solid-state nanopores in thin suspended dielectric membranes for various biomolecular sensing applications. On an unpatterned membrane, the site of nanopore formation by controlled breakdown is random. Nanopore formation on a specific site on the membrane has previously been realized using local thinning of the membrane by lithographic processes or laser-assisted photothermal etching under immersion in an aqueous salt solution. However, these approaches require elaborate and expensive cleanroom-based lithography processes or involve intricate procedures using custom-made equipment. Here, we present a rapid cleanroom-free approach using single pulse femtosecond laser exposures of 50 nm thick silicon nitride membranes in air to localize the site of nanopore formation by subsequent controlled breakdown to an area less than 500 nm in diameter on the membrane. The precise positioning of the nanopores on the membrane could be produced both using laser exposure powers which caused significant thinning of the silicon nitride membrane (up to 60% of the original thickness locally), as well as at laser powers which caused no visible modification of the membrane at all. We show that nanopores made using our approach can work as single-molecule sensors by performing dsDNA translocation experiments. Due to the applicability of femtosecond laser processing to a wide range of membrane materials, we expect our approach to simplify the fabrication of localized nanopores by controlled breakdown in a variety of thin film material stacks, thereby enabling more sophisticated nanopore sensors.", "doi": "10.1021/acsami.5c00255", "pmid": "39870574", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11803561"}], "notes": [], "created": "2026-08-20T08:08:29.746Z", "modified": "2026-08-21T11:34:56.490Z"}, {"entity": "publication", "iuid": "db35fa2c43da4f12accee901dc796a29", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/db35fa2c43da4f12accee901dc796a29.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/db35fa2c43da4f12accee901dc796a29"}}, "title": "In Situ Functionalization of Polar Polythiophene-Based Organic Electrochemical Transistor to Interface In Vitro Models.", "authors": [{"family": "Buchmann", "given": "Sebastian", "initials": "S", "orcid": "0000-0001-7442-3020", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d7fa19b55964898b15740e469f423a8.json"}}, {"family": "Stoop", "given": "Pepijn", "initials": "P"}, {"family": "Roekevisch", "given": "Kim", "initials": "K"}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Kroon", "given": "Renee", "initials": "R", "orcid": "0000-0001-8053-4288", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8edb30ab10ad4bf3b9122d9ca6f6380d.json"}}, {"family": "M\u00fcller", "given": "Christian", "initials": "C", "orcid": "0000-0001-7859-7909", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c72e3ff85aa545c0a6ddec76712541db.json"}}, {"family": "Hamedi", "given": "Mahiar M", "initials": "MM", "orcid": "0000-0001-9088-1064", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea65bd8c3fd642cbbd308e0641a0bc01.json"}}, {"family": "Zeglio", "given": "Erica", "initials": "E"}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}], "type": "journal article", "published": "2024-10-09", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "16", "issue": "40", "pages": "54292-54303", "issn-l": "1944-8244"}, "abstract": "Organic mixed ionic-electronic conductors are promising materials for interfacing and monitoring biological systems, with the aim of overcoming current challenges based on the mismatch between biological materials and convectional inorganic conductors. The conjugated polymer/polyelectrolyte complex poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT/PSS) is, up to date, the most widely used polymer for in vitro or in vivo measurements in the field of organic bioelectronics. However, PEDOT/PSS organic electrochemical transistors (OECTs) are limited by depletion mode operation and lack chemical groups that enable synthetic modifications for biointerfacing. Recently introduced thiophene-based polymers with oligoether side chains can operate in accumulation mode, and their chemical structure can be tuned during synthesis, for example, by the introduction of hydroxylated side chains. Here, we introduce a new thiophene-based conjugated polymer, p(g42T-T)-8% OH, where 8% of the glycol side chains are functionalized with a hydroxyl group. We report for the first time the compatibility of conjugated polymers containing ethylene glycol side chains in direct contact with cells. The additional hydroxyl group allows covalent modification of the surface of polymer films, enabling fine-tuning of the surface interaction properties of p(g42T-T)-8% OH with biological materials, either hindering or promoting cell adhesion. We further use p(g42T-T)-8% OH to fabricate the OECTs and demonstrate for the first time the monitoring of epithelial barrier formation of Caco-2 cells in vitro using accumulation mode OECTs. The conjugated polymer p(g42T-T)-8% OH allows organic-electronic-based materials to be easily modified and optimized to interface and monitor biological systems.", "doi": "10.1021/acsami.4c09197", "pmid": "39327895", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11472309"}], "notes": [], "created": "2026-08-20T08:08:22.084Z", "modified": "2026-08-21T11:34:52.536Z"}, {"entity": "publication", "iuid": "e154fdd25c074698958523cd700c4ae6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e154fdd25c074698958523cd700c4ae6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e154fdd25c074698958523cd700c4ae6"}}, "title": "Electromigrated Gold Nanogap Tunnel Junction Arrays: Fabrication and Electrical Behavior in Liquid and Gaseous Media.", "authors": [{"family": "Raja", "given": "Shyamprasad N", "initials": "SN", "orcid": "0000-0002-2278-1368", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/58df34262ccf4569b54162cfd38525be.json"}}, {"family": "Jain", "given": "Saumey", "initials": "S", "orcid": "0000-0002-2810-2151", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/969496e3da804b54bea1cf345294433f.json"}}, {"family": "Kipen", "given": "Javier", "initials": "J"}, {"family": "Jald\u00e9n", "given": "Joakim", "initials": "J"}, {"family": "Stemme", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9552-4234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/122f084eea624e5a801c8fafa6d48f6a.json"}}, {"family": "Herland", "given": "Anna", "initials": "A", "orcid": "0000-0002-5002-2537", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/367ed0d139fe4136808b62ee61baa4f0.json"}}, {"family": "Niklaus", "given": "Frank", "initials": "F", "orcid": "0000-0002-0525-8647", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cbba7fd047e8468288fc79b7b0275ff3.json"}}], "type": "journal article", "published": "2024-07-17", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "16", "issue": "28", "pages": "37131-37146", "issn-l": "1944-8244"}, "abstract": "Tunnel junctions have been suggested as high-throughput electronic single molecule sensors in liquids with several seminal experiments conducted using break junctions with reconfigurable gaps. For practical single molecule sensing applications, arrays of on-chip integrated fixed-gap tunnel junctions that can be built into compact systems are preferable. Fabricating nanogaps by electromigration is one of the most promising approaches to realize on-chip integrated tunnel junction sensors. However, the electrical behavior of fixed-gap tunnel junctions immersed in liquid media has not been systematically studied to date, and the formation of electromigrated nanogap tunnel junctions in liquid media has not yet been demonstrated. In this work, we perform a comparative study of the formation and electrical behavior of arrays of gold nanogap tunnel junctions made by feedback-controlled electromigration immersed in various liquid and gaseous media (deionized water, mesitylene, ethanol, nitrogen, and air). We demonstrate that tunnel junctions can be obtained from microfabricated gold nanoconstrictions inside liquid media. Electromigration of junctions in air produces the highest yield (61-67%), electromigration in deionized water and mesitylene results in a lower yield than in air (44-48%), whereas electromigration in ethanol fails to produce viable tunnel junctions due to interfering electrochemical processes. We map out the stability of the conductance characteristics of the resulting tunnel junctions and identify medium-specific operational conditions that have an impact on the yield of forming stable junctions. Furthermore, we highlight the unique challenges associated with working with arrays of large numbers of tunnel junctions in batches. Our findings will inform future efforts to build single molecule sensors using on-chip integrated tunnel junctions.", "doi": "10.1021/acsami.4c03282", "pmid": "38954436", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11261569"}], "notes": [], "created": "2026-08-20T08:08:20.105Z", "modified": "2026-08-21T11:34:50.541Z"}, {"entity": "publication", "iuid": "3851d48fb20e4cf5bb4345022ce2719b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3851d48fb20e4cf5bb4345022ce2719b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3851d48fb20e4cf5bb4345022ce2719b"}}, "title": "Two-Photon Polymerization Printing with High Metal Nanoparticle Loading.", "authors": [{"family": "Kilic", "given": "Nuzhet I", "initials": "NI", "orcid": "0000-0002-9201-0454", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d6538213423450fb3ea2852390a5ba3.json"}}, {"family": "Saladino", "given": "Giovanni M", "initials": "GM", "orcid": "0000-0002-6854-1423", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc38851d89384266a94e44f1aa016e3f.json"}}, {"family": "Johansson", "given": "Sofia", "initials": "S", "orcid": "0000-0003-3672-9883", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d976bdc17d7248d6b60c4f7a47fc84c7.json"}}, {"family": "Shen", "given": "Rickard", "initials": "R", "orcid": "0000-0002-2641-7838", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/010ce4a4c3394992bb37523220cee901.json"}}, {"family": "McDorman", "given": "Cacie", "initials": "C"}, {"family": "Toprak", "given": "Muhammet S", "initials": "MS", "orcid": "0000-0001-5678-5298", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0fc4640233c142109ac161e3b137dcc0.json"}}, {"family": "Johansson", "given": "Stefan", "initials": "S"}], "type": "journal article", "published": "2023-10-25", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "15", "issue": "42", "pages": "49794-49804", "issn-l": "1944-8244"}, "abstract": "Two-photon polymerization (2PP) is an efficient technique to achieve high-resolution, three-dimensional (3D)-printed complex structures. However, it is restricted to photocurable monomer combinations, thus presenting constraints when aiming at attaining functionally active resist formulations and structures. In this context, metal nanoparticle (NP) integration as an additive can enable functionality and pave the way to more dedicated applications. Challenges lay on the maximum NP concentrations that can be incorporated into photocurable resist formulations due to the laser-triggered interactions, which primarily originate from laser scattering and absorption, as well as the limited dispersibility threshold. In this study, we propose an approach to address these two constraints by integrating metallic Rh NPs formed ex situ, purposely designed for this scope. The absence of surface plasmon resonance (SPR) within the visible and near-infrared spectra, coupled with the limited absorption value measured at the laser operating wavelength (780 nm), significantly limits the laser-induced interactions. Moreover, the dispersibility threshold is increased by engineering the NP surface to be compatible with the photocurable resin, permitting us to achieve concentrations of up to 2 wt %, which, to our knowledge, is significantly higher than the previously reported limit (or threshold) for embedded metal NPs. Another distinctive advantage of employing Rh NPs is their role as promising contrast agents for X-ray fluorescence (XRF) bioimaging. We demonstrated the presence of Rh NPs within the whole 2PP-printed structure and emphasized the potential use of NP-loaded 3D-printed nanostructures for medical devices.", "doi": "10.1021/acsami.3c10581", "pmid": "37816209", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10614202"}], "notes": [], "created": "2026-08-21T11:34:48.476Z", "modified": "2026-08-21T11:34:48.641Z"}, {"entity": "publication", "iuid": "842f5e3273af43e3a5a52fb26ac1ed5e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/842f5e3273af43e3a5a52fb26ac1ed5e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/842f5e3273af43e3a5a52fb26ac1ed5e"}}, "title": "Hyperthermia-Induced In Situ Drug Amorphization by Superparamagnetic Nanoparticles in Oral Dosage Forms.", "authors": [{"family": "Ansari", "given": "Shaquib Rahman", "initials": "SR", "orcid": "0000-0003-3710-405X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d50cab7c722144d185a212d83600d80b.json"}}, {"family": "Hempel", "given": "Nele-Johanna", "initials": "NJ"}, {"family": "Asad", "given": "Shno", "initials": "S"}, {"family": "Svedlindh", "given": "Peter", "initials": "P", "orcid": "0000-0002-3049-6831", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b0341fe5a8e2404f89e556908fdeca81.json"}}, {"family": "Bergstr\u00f6m", "given": "Christel A S", "initials": "CAS", "orcid": "0000-0002-8917-2612", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bae48739770b4614bc6f64eab574dd75.json"}}, {"family": "L\u00f6bmann", "given": "Korbinian", "initials": "K", "orcid": "0000-0002-8710-6347", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed940056bb8248468f21964dba464f49.json"}}, {"family": "Teleki", "given": "Alexandra", "initials": "A", "orcid": "0000-0001-6514-8960", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6fe98cb60a304a5aa33e24401fcd8af6.json"}}], "type": "journal article", "published": "2022-05-18", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "14", "issue": "19", "pages": "21978-21988", "issn-l": "1944-8244"}, "abstract": "Superparamagnetic iron oxide nanoparticles (SPIONs) generate heat upon exposure to an alternating magnetic field (AMF), which has been studied for hyperthermia treatment and triggered drug release. This study introduces a novel application of magnetic hyperthermia to induce amorphization of a poorly aqueous soluble drug, celecoxib, in situ in tablets for oral administration. Poor aqueous solubility of many drug candidates is a major hurdle in oral drug development. A novel approach to overcome this challenge is in situ amorphization of crystalline drugs. This method facilitates amorphization by molecular dispersion of the drug in a polymeric network inside a tablet, circumventing the physical instability encountered during the manufacturing and storage of conventional amorphous solid dispersions. However, the current shortcomings of this approach include low drug loading, toxicity of excipients, and drug degradation. Here, doped SPIONs produced by flame spray pyrolysis are compacted with polyvinylpyrrolidone and celecoxib and exposed to an AMF in solid state. A design of experiments approach was used to investigate the effects of SPION composition (Zn0.5Fe2.5O4 and Mn0.5Fe2.5O4), doped SPION content (10-20 wt %), drug load (30-50 wt %), and duration of AMF (3-15 min) on the degree of drug amorphization. The degree of amorphization is strongly linked to the maximum tablet temperature achieved during the AMF exposure (r = 0.96), which depends on the SPION composition and content in the tablets. Complete amorphization is achieved with 20 wt % Mn0.5Fe2.5O4 and 30 wt % celecoxib in the tablets that reached the maximum temperature of 165.2 \u00b0C after 15 min of AMF exposure. Furthermore, manganese ferrite exhibits no toxicity in human intestinal Caco-2 cell lines. The resulting maximum solubility of in situ amorphized celecoxib is 5 times higher than that of crystalline celecoxib in biorelevant intestinal fluid. This demonstrates the promising capability of SPIONs as enabling excipients to magnetically induce amorphization in situ in oral dosage forms.", "doi": "10.1021/acsami.2c03556", "pmid": "35452221", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9121342"}], "notes": [], "created": "2022-12-01T20:52:30.424Z", "modified": "2026-08-21T11:34:46.129Z"}, {"entity": "publication", "iuid": "0517ee68ec234694b34b1dec5fe4e8eb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0517ee68ec234694b34b1dec5fe4e8eb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0517ee68ec234694b34b1dec5fe4e8eb"}}, "title": "Inhibition of Tumor-Host Cell Interactions Using Synthetic Heparin Mimetics.", "authors": [{"family": "Gockel", "given": "Lukas M", "initials": "LM"}, {"family": "Heyes", "given": "Martin", "initials": "M"}, {"family": "Li", "given": "Honglian", "initials": "H"}, {"family": "Al Nahain", "given": "Abdullah", "initials": "A"}, {"family": "Gorzelanny", "given": "Christian", "initials": "C"}, {"family": "Schlesinger", "given": "Martin", "initials": "M"}, {"family": "Holdenrieder", "given": "Stefan", "initials": "S"}, {"family": "Li", "given": "Jin-Ping", "initials": "JP"}, {"family": "Ferro", "given": "Vito", "initials": "V", "orcid": "0000-0003-3306-2550", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea823123e5964b6b93884bfc88d7905e.json"}}, {"family": "Bendas", "given": "Gerd", "initials": "G", "orcid": "0000-0002-8667-7201", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04a4b627b32e466ba5d83fc8b1a8691f.json"}}], "type": "journal article", "published": "2021-02-17", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "13", "issue": "6", "pages": "7080-7093", "issn-l": "1944-8244"}, "abstract": "Low-molecular-weight heparin (LMWH) is the guideline-based drug for antithrombotic treatment of cancer patients, while its direct antitumor effects are a matter of ongoing debate. Although therapeutically established for decades, LMWH has several drawbacks mainly associated with its origin from animal sources. Aiming to overcome these limitations, a library of synthetic heparin mimetic polymers consisting of homo- and copolymers of sulfonated and carboxylated noncarbohydrate monomers has recently been synthesized via reversible addition-fragmentation chain transfer polymerization. These heparin mimetics were investigated for their capacities to interfere with simulated steps of tumor cell metastasis. Among them, homo- and copolymers from sodium 4-styrenesulfonate (poly(SSS)) with acrylic acid (poly(SSS-co-AA)) with an MW between 5 and 50 kDa efficiently attenuated cancer cell-induced coagulation and thus platelet activation and degranulation similar to or even better than LMWH. Furthermore, independent of anticoagulant activities, these polymers affected other metastasis-relevant targets with impressive affinities. Hence, they blocked heparanase enzymatic activity outmatching commercial heparins or a glycosidic drug candidate. Furthermore, these polymers bind P-selectin and the integrin VLA-4 similar to or even better than heparin, indicated by a biosensor approach and thus efficiently blocked melanoma cell binding to endothelium under blood flow conditions. This is the first report on the prospects of synthetic heparin mimetics as promising nontoxic compounds in oncology to potentially substitute heparin as an anticoagulant and to better understand its role as an antimetastatic drug.", "doi": "10.1021/acsami.0c20744", "pmid": "33533245", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:34:44.200Z", "modified": "2026-08-21T11:34:44.329Z"}, {"entity": "publication", "iuid": "b3bb0b8495c44f19bf6423851c0e1547", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b3bb0b8495c44f19bf6423851c0e1547.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b3bb0b8495c44f19bf6423851c0e1547"}}, "title": "Porous Cellulose Nanofiber-Based Microcapsules for Biomolecular Sensing.", "authors": [{"family": "Paulraj", "given": "Thomas", "initials": "T", "orcid": "0000-0001-5098-3525", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b0bb7dbf6844a8bb76feabf638f857f.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S"}, {"family": "Riazanova", "given": "Anastasia V", "initials": "AV"}, {"family": "Wu", "given": "Qiong", "initials": "Q"}, {"family": "Crespo", "given": "Gaston A", "initials": "GA", "orcid": "0000-0002-1221-3906", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/882e4bc0ef9145b098a493c764f4bd6e.json"}}, {"family": "Svagan", "given": "Anna J", "initials": "AJ", "orcid": "0000-0002-4583-723X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d71694480999460b924aac5b2e25d4b7.json"}}], "type": "journal article", "published": "2018-12-05", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "10", "issue": "48", "pages": "41146-41154", "issn-l": "1944-8244"}, "abstract": "Cellulose nanofibers (CNFs) have recently attracted a lot of attention in sensing because of their multifunctional character and properties such as renewability, nontoxicity, biodegradability, printability, and optical transparency in addition to unique physicochemical, barrier, and mechanical properties. However, the focus has exclusively been devoted toward developing two-dimensional sensing platforms in the form of nanopaper or nanocellulose-based hydrogels. To improve the flexibility and sensing performance in situ, for example, to detect biomarkers in vivo for early disease diagnostics, more advanced CNF-based structures are needed. Here, we developed porous and hollow, yet robust, CNF-based microcapsules using only the primary plant cell wall components, CNF, pectin, and xyloglucan, to assemble the capsule wall. The fluorescein isothiocyanate-labeled dextrans with MW of 70 and 2000 kDa could enter the hollow capsules at a rate of 0.13 \u00b1 0.04 and 0.014 \u00b1 0.009 s-1, respectively. This property is very attractive because it minimizes the influence of mass transport through the capsule wall on the response time. As a proof of concept, glucose oxidase (GOx) enzyme was loaded (and cross-linked) in the microcapsule interior with an encapsulation efficiency of 68 \u00b1 2%. The GOx-loaded microcapsules were immobilized on a variety of surfaces (here, inside a flow channel, on a carbon-coated sensor or a graphite rod) and glucose concentrations up to 10 mM could successfully be measured. The present concept offers new opportunities in the development of simple, more efficient, and disposable nanocellulose-based analytical devices for several sensing applications including environmental monitoring, healthcare, and diagnostics.", "doi": "10.1021/acsami.8b16058", "pmid": "30412378", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:35:00.463Z", "modified": "2026-08-21T11:35:00.615Z"}, {"entity": "publication", "iuid": "b6530009f52d4a99983bbf8042bd296c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b6530009f52d4a99983bbf8042bd296c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b6530009f52d4a99983bbf8042bd296c"}}, "title": "Multifunctional Hyaluronic Acid and Chondroitin Sulfate Nanoparticles: Impact of Glycosaminoglycan Presentation on Receptor Mediated Cellular Uptake and Immune Activation.", "authors": [{"family": "Oommen", "given": "Oommen P", "initials": "OP"}, {"family": "Duehrkop", "given": "Claudia", "initials": "C"}, {"family": "Nilsson", "given": "Bo", "initials": "B"}, {"family": "Hilborn", "given": "J\u00f6ns", "initials": "J"}, {"family": "Varghese", "given": "Oommen P", "initials": "OP"}], "type": "journal article", "published": "2016-08-17", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "8", "issue": "32", "pages": "20614-20624", "issn-l": "1944-8244"}, "abstract": "Hyaluronic acid (HA) and chondroitin sulfate (CS) polymers are extensively used for various biomedical applications, such as for tissue engineering, drug delivery, and gene delivery. Although both these biopolymers are known to target cell surface CD44 receptors, their relative cellular targeting properties and immune activation potential have never been evaluated. In this article, we present the synthesis and characterization of novel self-assembled supramolecular HA and CS nanoparticles (NPs). These NPs were developed using fluorescein as a hydrophobic component that induced amphiphilicity in biopolymers and also efficiently stabilized anticancer drug doxorubicin (DOX) promoting a near zero-order drug release. The cellular uptake and cytotoxicity studies of these NPs in different human cancer lines, namely, human colorectal carcinoma cell line HCT116 and human breast cancer cell line MCF-7 demonstrated dose dependent cytotoxicity. Interestingly, both NPs showed CD44 dependent cellular uptake with the CS-DOX NP displaying higher dose-dependent cytotoxicity than the HA-DOX NP in different mammalian cells tested. Immunological evaluation of these nanocarriers in an ex vivo human whole blood model revealed that unlike unmodified polymers, the HA NP and CS NP surprisingly showed platelet aggregation and thrombin-antithrombin complex formation at high concentrations (0.8 mg/mL). We also observed a clear difference in early- and late-stage complement activation (C3a and sC5b-9) with CS and CS NP triggering significant complement activation at high concentrations (0.08-0.8 mg/mL), unlike HA and HA NP. These results offer new insight into designing glycosaminoglycan-based NPs and understanding their hematological responses and targeting ability.", "doi": "10.1021/acsami.6b06823", "pmid": "27468113", "labels": [], "xrefs": [], "notes": [], "created": "2018-12-05T10:02:28.707Z", "modified": "2026-08-21T11:34:58.568Z"}, {"entity": "publication", "iuid": "2fe249e10e5b460cbec1cc1fd7c0b207", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2fe249e10e5b460cbec1cc1fd7c0b207.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2fe249e10e5b460cbec1cc1fd7c0b207"}}, "title": "Hydrogel patterning by diffusion through the matrix and subsequent light-triggered chemical immobilization.", "authors": [{"family": "Yi", "given": "Zheyi", "initials": "Z"}, {"family": "Zhang", "given": "Yu", "initials": "Y"}, {"family": "Kootala", "given": "Sujit", "initials": "S"}, {"family": "Hilborn", "given": "J\u00f6ns", "initials": "J"}, {"family": "Ossipov", "given": "Dmitri A", "initials": "DA"}], "type": "journal article", "published": "2015-01-21", "journal": {"title": "ACS Appl Mater Interfaces", "issn": "1944-8252", "volume": "7", "issue": "2", "pages": "1194-1206", "issn-l": "1944-8244"}, "abstract": "A novel approach to hyaluronic acid (HA) hydrogel with a chemical gradient of the matrix-linked bisphosphonate (BP) groups is presented. The method consists of two steps, including initial generation of physical gradient patterns of BPs by diffusion of BP acrylamide reagent into HA matrix carrying thiol groups and subsequent chemical immobilization of the BP groups by UV light-triggered thiol-ene addition reaction. This gradient hydrogel permits spatial three-dimensional regulation of secondary interactions of different molecules with the polymer matrix. In particular, graded amounts of cytochrome c (cyt c) were reversibly absorbed in the hydrogel, thus enabling the subsequent spatially controlled release of the therapeutic protein. The obtained patterned hydrogel acts also as a unique reactor in which peroxidase-catalyzed oxidation of a substrate is determined by spatial position of the enzyme (cyt c) in the matrix resulting in a range of product concentrations. As an example, matrix template-assisted oxidation of 3,3',5,5'-tetarmethylbenzydine (TMB) in the presence of H2O2 occurs simultaneously at different rates within the gradient hydrogel. Moreover, calcium binding to the gradient HABP hydrogel reflects the pattern of immobilized BP groups eventually leading to the graded biomineralization of the matrix. This approach opens new possibilities for use of hydrogels as dynamic models for biologic three-dimensional structures such as extracellular matrix.", "doi": "10.1021/am506926w", "pmid": "25575380", "labels": [], "xrefs": [], "notes": [], "created": "2018-12-05T11:32:01.842Z", "modified": "2026-08-21T11:37:07.775Z"}], "created": "2018-12-05T10:02:28.721Z", "modified": "2020-11-27T13:12:58.475Z"}